Journal:

Article Title: Human Cytomegalovirus UL99-Encoded pp28 Is Required for the Cytoplasmic Envelopment of Tegument-Associated Capsids

doi: 10.1128/JVI.77.19.10594-10605.2003

Figure Lengend Snippet: Accumulation of viral proteins after infection with wild-type (wt) and pp28-deficient viruses. Normal fibroblasts were mock-infected (M) or infected with BADwt, BADsubUL99, or BADpmUL99 at a multiplicity of infection of 0.01 PFU/cell, and whole-cell extracts were prepared at 72 h (A) or at the indicated times after infection (days postinfection [dpi]) (B). Proteins were analyzed by Western blot analysis with antibodies specific for the UL123-encoded IE1, pUL97, UL83-encoded pp65, UL82-encoded pp71, pUL44, or UL99-encoded pp28.

Article Snippet: Primary antibodies used in these studies were UL32-encoded pp150 and UL83-encoded pp65 rabbit polyclonal antibodies (gift of M. Schrader, University of Marburg), UL99-encoded pp28 mouse monoclonal antibody (10B4-29), UL55-encoded gB (58-15 monoclonal antibody produced by immunization with disrupted virions) (P. Robinson and T. Shenk, unpublished data).

Techniques: Infection, Western Blot

Journal:

Article Title: Human Cytomegalovirus UL99-Encoded pp28 Is Required for the Cytoplasmic Envelopment of Tegument-Associated Capsids

doi: 10.1128/JVI.77.19.10594-10605.2003

Figure Lengend Snippet: Localization of viral proteins expressed in normal fibroblasts infected with wild-type (WT) and pp28-deficient viruses. Cells were infected with BADwt or BADsubUL99 at a multiplicity of infection of 0.01 PFU/cell and processed for immunofluorescence at 120 h postinfection. Four images are presented for each cell. The upper-left quadrant shows UL32-encoded pp150 (A), UL83-encoded pp65 (B), or UL55-encoded gB (C) in red. The lower-left quadrants of panels A and B display pp28 in blue, whereas the lower-left panel of C contains blue-stained nuclei. In all panels, the upper-right quadrant displays Golgi in green and the lower-right panel shows a multicolored merged image.

Article Snippet: Primary antibodies used in these studies were UL32-encoded pp150 and UL83-encoded pp65 rabbit polyclonal antibodies (gift of M. Schrader, University of Marburg), UL99-encoded pp28 mouse monoclonal antibody (10B4-29), UL55-encoded gB (58-15 monoclonal antibody produced by immunization with disrupted virions) (P. Robinson and T. Shenk, unpublished data).

Techniques: Infection, Immunofluorescence, Staining

Journal: International journal of molecular sciences

Article Title: WGA-M001, a Mixture of Total Extracts of Tagetes erecta and Ocimum basilicum , Synergistically Alleviates Cartilage Destruction by Inhibiting ERK and NF-κB Signaling.

doi: 10.3390/ijms242417459

Figure Lengend Snippet: Figure 4. WGA-M001 regulated OA-related molecules through dephosphorylation in the NF-κB and ERK pathways. For in silico analysis, 200 µg/mL of Tagetes erecta, Ocimum basilicum, or WGA-M001 was used for treatment of chondrocytes for 12 h along with IL-1β (1 ng/mL), and RNA sequencing was performed. (A) In each signaling pathway, the number of genes upregulated by IL-1β and then downregulated by T. erecta, O. basilicum, or WGA-M001 was shown. (B,C) Protein levels of pp38, p38, pJNK, JNK, pERK, ERK, pp65, p65, and IκB were detected by Western blot analysis and relative intensities were quantified by densitometry (n = 5). Each protein level was normalized to ERK. Data are represented as mean ± SD as results of analysis by one-way ANOVA with Dunnett’s multiple comparisons test (n = 5). * p < 0.05, **** p < 0.0001, and n.s = not significant.

Article Snippet: 2023, 24, 17459 12 of 16 cam), rabbit anti-COX-2 (ab52237; Abcam), mouse anti-IκB (9242; Cell Signaling Technology (CST), Danvers, MA, USA), mouse anti-p65 (#6956; CST), mouse anti-pp65 (#13346; CST), mouse anti-p38 (#9212; CST), mouse anti-pp38 (#9215S; CST), mouse anti-c-JNK (#9252S; CST), mouse anti-pJNK (#9251S; CST), and mouse anti-pERK (#9101S; CST).

Techniques: De-Phosphorylation Assay, In Silico, RNA Sequencing, Western Blot

Journal: Journal of Food Biochemistry

Article Title: Betulinic Acid–Mediated NR1D1 Alleviates Dextran Sulfate Sodium–Induced Colitis in Mice by Modulating VDAC1/NF‐κВ

doi: 10.1155/jfbc/3244032

Figure Lengend Snippet: Figure 4: BHA alleviates infammatory response in DSS-induced colitis. (a) Te gene expression of IL-1β, IL-6, TNF-α, and IL-18. (b) Te content of IL-1β and IL-18 in colon. (c) NR1D1, VDAC1, p65, and pp65 gray values. (d) Te protein of NR1D1 and VDAC1 and the ratio of pp65 to p65. Data were analyzed by using one-way ANOVA followed by the Duncan’s test. Te signifcance indicated refers to the comparison between controls and all treatment groups.

Article Snippet: Te primary antibodies applied included NR1D1 (WH0009572M2, Sigma-Aldrich, MO), VDAC1 (ab306581, Abcam, Cambridge, UK), pp65 (3033, CST, China), p65 (8242, CST, China), and β-actin (ab8226, Abcam, Cambridge, UK), following the manufacturers’ instructions.

Techniques: Gene Expression, Comparison

Journal: Journal of Food Biochemistry

Article Title: Betulinic Acid–Mediated NR1D1 Alleviates Dextran Sulfate Sodium–Induced Colitis in Mice by Modulating VDAC1/NF‐κВ

doi: 10.1155/jfbc/3244032

Figure Lengend Snippet: Figure 7: Efect of BHA on infammatory response in LPS-induced CCD841. (a) Te gene expression of IL-1β, IL-6, TNF-α and IL-18. (b) Te content of IL-1β and IL-18. (c) NR1D1, VDAC1, p65, and pp65 gray values. (d) Te protein of NR1D1 and VDAC1 and the ratio of pp65 to p65. (e) Immunofuorescence of NR1D1. Data were analyzed by using one-way ANOVA followed by the Duncan’s test. Te signifcance indicated refers to the comparison between controls and all treatment groups.

Article Snippet: Te primary antibodies applied included NR1D1 (WH0009572M2, Sigma-Aldrich, MO), VDAC1 (ab306581, Abcam, Cambridge, UK), pp65 (3033, CST, China), p65 (8242, CST, China), and β-actin (ab8226, Abcam, Cambridge, UK), following the manufacturers’ instructions.

Techniques: Gene Expression, Comparison

Journal: Journal of Food Biochemistry

Article Title: Betulinic Acid–Mediated NR1D1 Alleviates Dextran Sulfate Sodium–Induced Colitis in Mice by Modulating VDAC1/NF‐κВ

doi: 10.1155/jfbc/3244032

Figure Lengend Snippet: Figure 9: Efect of NR1D1 on genes and proteins related to infammation. Generation of NR1D1 knockdown cell using siRNA. (a) NR1D1 gray values. (b) NR1D1 protein expression levels. (c) Te genes expression of IL-1β, IL-6, TNF-α and IL-18. (d) VDAC1, p65, and pp65 gray values. (e) Te protein of VDAC1 and the ratio of pp65 to p65. Data were analyzed by using one-way ANOVA followed by the Duncan’s test. Te signifcance indicated refers to the comparison between controls and all treatment groups.

Article Snippet: Te primary antibodies applied included NR1D1 (WH0009572M2, Sigma-Aldrich, MO), VDAC1 (ab306581, Abcam, Cambridge, UK), pp65 (3033, CST, China), p65 (8242, CST, China), and β-actin (ab8226, Abcam, Cambridge, UK), following the manufacturers’ instructions.

Techniques: Knockdown, Expressing, Comparison

Journal: Journal of Inflammation Research

Article Title: PM2.5 Promotes Macrophage-Mediated Inflammatory Response Through Airway Epithelial Cell-Derived Exosomal miR-155-5p

doi: 10.2147/jir.s482509

Figure Lengend Snippet: Figure 1 PM2.5-BEAS-2B co-cultured with Mφ. (A) PM2.5-BEAS-2B cell co-cultured with Mφ mode diagram; (B) Cellular supernatant IL-6, IL-1β, TNF-α contents of Mφ co-cultured with PM2.5-BEAS-2B cells; (C) mRNA expression levels of IL-6, IL-1β, TNF-α in Mφ co-cultured with PM2.5-BEAS-2B cells; (D) miR-155-5p expression level in Mφ co-cultured with PM2.5-BEAS-2B cells; (E) mRNA expression levels of SOCS1, NF-κB in Mφ co-cultured with PM2.5-BEAS-2B cells; (F and G) Protein expression level of SOCS1, P65, PP65 in Mφ co-cultured with PM2.5-BEAS-2B cells as detected by Western blot analysis as well as statistical analysis of gray values. Experimental group: group A PM2.50-BEAS-2B+Mφ, group B PM2.5100-BEAS-2B+Mφ, group C PM2.5200-BEAS-2B+Mφ, group D PM2.5400-BEAS-2B+Mφ. **P<0.01, *P<0.05 vs group A.

Article Snippet: After blocking with protein-free rapid-blocking solution (Boster, Wuhan, China) for 1h, the membranes were exposed to rabbit anti-SOCS1 polyclonal antibody (Boster), rabbit anti-P65 polyclonal antibody (Boster), rabbit anti-PP65 monoclonal antibody (CellSignalingTechnology), rabbit anti-βtublin polyclonal antibody (Boster), and rabbit anti-GAPDH polyclonal antibody (Boster) at 4°C overnight.

Techniques: Cell Culture, Expressing, Western Blot

Journal: Journal of Inflammation Research

Article Title: PM2.5 Promotes Macrophage-Mediated Inflammatory Response Through Airway Epithelial Cell-Derived Exosomal miR-155-5p

doi: 10.2147/jir.s482509

Figure Lengend Snippet: Figure 4 PM2.5-BEAS-2B-Exo co-cultured with Mφ. (A) PM2.5-BEAS-2B-Exo co-cultured with Mφ mode diagram; (B) Cellular supernatant IL-6, IL-1β, TNF-α contents of Mφ incubated with PM2.5-BEAS-2B-Exo; (C) mRNA expression levels of IL-6, IL-1β, TNF-α in Mφ incubated with PM2.5-BEAS-2B-Exo; (D) miR-155-5p expression level in Mφ incubated with PM2.5-BEAS-2B-Exo; (E) mRNA expression levels of SOCS1, NF-κB in Mφ incubated with PM2.5-BEAS-2B-Exo; (F and G) Protein expression level of SOCS1, P65, PP65 in Mφ incubated with PM2.5-BEAS-2B-Exo as detected by Western blot analysis as well as statistical analysis of gray values. Experimental group: group E PM2.50-BEAS-2B-Exo+Mφ, group F PM2.5100-BEAS-2B-Exo+Mφ, group G PM2.5200-BEAS-2B-Exo+Mφ, group H PM2.5400-BEAS-2B-Exo+Mφ. **P<0.01, *P<0.05 vs group E.

Article Snippet: After blocking with protein-free rapid-blocking solution (Boster, Wuhan, China) for 1h, the membranes were exposed to rabbit anti-SOCS1 polyclonal antibody (Boster), rabbit anti-P65 polyclonal antibody (Boster), rabbit anti-PP65 monoclonal antibody (CellSignalingTechnology), rabbit anti-βtublin polyclonal antibody (Boster), and rabbit anti-GAPDH polyclonal antibody (Boster) at 4°C overnight.

Techniques: Cell Culture, Incubation, Expressing, Western Blot

Journal: Journal of Inflammation Research

Article Title: PM2.5 Promotes Macrophage-Mediated Inflammatory Response Through Airway Epithelial Cell-Derived Exosomal miR-155-5p

doi: 10.2147/jir.s482509

Figure Lengend Snippet: Figure 5 GW4869 improves inflammation in Mφ. PM2.5-BEAS-2B cells were pretreated with GW4869 and co-cultured with Mφ. (A) Cellular supernatant IL-6, IL-1β, TNF-α contents of Mφ; (B) mRNA expression levels of IL-6, IL-1β, TNF-α in Mφ; (C) miR-155-5p expression level in Mφ; (D) mRNA expression levels of SOCS1, NF-κB in Mφ; (E and F) Protein expression level of SOCS1, P65, PP65 in Mφ as detected by Western blot analysis as well as statistical analysis of gray values. Experimental group: group A PM2.50-BEAS-2B+Mφ, group C PM2.5200-BEAS-2B+Mφ, group I PM2.5200-BEAS-2B+GW4869+Mφ. **P<0.01, *P<0.05 vs group A;▲▲P<0.01,▲P<0.05 vs group C.

Article Snippet: After blocking with protein-free rapid-blocking solution (Boster, Wuhan, China) for 1h, the membranes were exposed to rabbit anti-SOCS1 polyclonal antibody (Boster), rabbit anti-P65 polyclonal antibody (Boster), rabbit anti-PP65 monoclonal antibody (CellSignalingTechnology), rabbit anti-βtublin polyclonal antibody (Boster), and rabbit anti-GAPDH polyclonal antibody (Boster) at 4°C overnight.

Techniques: Cell Culture, Expressing, Western Blot

Journal: Virology Journal

Article Title: In vitro study on human cytomegalovirus affecting early pregnancy villous EVT's invasion function

doi: 10.1186/1743-422X-8-114

Figure Lengend Snippet: Histology of two groups'villous explants under HE staining . A. Human villous explants in vitro HE ×200; B. Human villous explants infected by HCMV in vitro HE ×200.

Article Snippet: DMEM/Ham's F 12 culture medium (Gibco), standard fetal bovine serum (Hyclone), rabbit anti HCMV pp65 polyclonal antibody (Santa), rabbit anti c-erbB-2 polyclonal antibody, rabbit anti MMP-2 and MMP-9 polyclonal antibodies (Wuhan Boster Bio-engineering Co., Ltd), SP immunohistochemistry kit (Zhongshan Goldenbridge Biotechnology Co., Ltd), total protein extraction kit (BestBio), β-actin loading control antibody (Beijing Biosynthesis Biotechnology Co., Ltd) and so on.

Techniques: Staining, In Vitro, Infection

Journal: Virology Journal

Article Title: In vitro study on human cytomegalovirus affecting early pregnancy villous EVT's invasion function

doi: 10.1186/1743-422X-8-114

Figure Lengend Snippet: The expression of HCMVpp65 antigen in two groups'villous explants detected by immunohistostaining . A. The expression of HCMVpp65 antigen in normal human villous explants detected by immunohistostaining ×400; B. The expression of HCMVpp65 antigen in human villous explants infected by HCMV detected by immunohistostaining ×400.

Article Snippet: DMEM/Ham's F 12 culture medium (Gibco), standard fetal bovine serum (Hyclone), rabbit anti HCMV pp65 polyclonal antibody (Santa), rabbit anti c-erbB-2 polyclonal antibody, rabbit anti MMP-2 and MMP-9 polyclonal antibodies (Wuhan Boster Bio-engineering Co., Ltd), SP immunohistochemistry kit (Zhongshan Goldenbridge Biotechnology Co., Ltd), total protein extraction kit (BestBio), β-actin loading control antibody (Beijing Biosynthesis Biotechnology Co., Ltd) and so on.

Techniques: Expressing, Infection